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KMID : 0376219850220040739
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Chonnam Medical Journal
1985 Volume.22 No. 4 p.739 ~ p.746
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Effect of Light on the Formation of Superoxide Radical by Phenazine Methosuifate
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李民化/Lee, Min-wha
李起榮/梁誠烈/朴昞柱/全鉉權/Lee, Kee-yung/Yang, Sung-yeui/Park, Byong-Ju/Chun, Hyun-kwon
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Abstract
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The light-induced formation of superoxide radical(02) by the reaction of phenazine methosulfate(PMS) with oxygen was demonstrated by the measurement of oxygen consumption during the reaction.
In the dark, the oxygen consumption by PMS alone did not occur, but when NADH was added, it occurred rapidly. Superoxide dismutase(SOD), catalase and peroxides inhibited the oxygen consumption by PMS+NADH system in the dark. Nitroblue tetrazolium(NBT) also inhibited the oxygen consumption, indicating that NBT acted as a scavenger of 0z- formed from PMS+NADH system. On exposure to the room light or to ultraviolet light, the PMS solution consumed oxygen rapidly with the production of hydrogen ion even in the absence of NADH. Catalase and peroxides inhibited the oxygen consumption by PMS "in the light as in the case of the PMS+NADH in the dark, but SOD was without effect.
Based on these results, the reaction mechanism by which the photolysis of PMS under aerobic condition produces superoxide radical is proposed. It is also suggested that superoxide radicals can rapidly dispute to hydrogen peroxide and oxygen under the influence of light.
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